Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/224177
Beta-blocker treatment of patients with atrial fibrillation attenuates abnormal electrical activity induced by spontaneous calcium release
Títol de la revista
ISSN de la revista
Títol del volum
Resum
Aims: Atrial fibrillation (AF) has been associated with excessive spontaneous calcium release, linked to cyclic AMP (cAMP)-dependent phosphorylation of calcium regulatory proteins. Because β-blockers are expected to attenuate cAMP-dependent signaling, we aimed to examine whether the treatment of patients with β-blockers affected the incidence of spontaneous calcium release events or transient inward currents (ITI). Methods: The impact of treatment with commonly used β-blockers was analyzed in human atrial myocytes from 371 patients using patch-clamp technique, confocal calcium imaging or immunofluorescent labeling. Data were analyzed using multivariate regression analysis taking into account potentially confounding effects of relevant clinical factors RESULTS: The L-type calcium current (ICa) density was diminished significantly in patients with chronic but not paroxysmal AF and the treatment of patients with β-blockers did not affect ICa density in any group. By contrast, the ITI frequency was elevated in patients with either paroxysmal or chronic AF that did not receive treatment, and β-blocker treatment reduced the frequency to levels observed in patients without AF. Confocal calcium imaging showed that β-blocker treatment also reduced the calcium spark frequency in patients with AF to levels observed in those without AF. Furthermore, phosphorylation of the ryanodine receptor (RyR2) at Ser-2808 and phospholamban at Ser-16 was significantly lower in patients with AF that received β-blockers. Conclusion: Together, our findings demonstrate that β-blocker treatment may be of therapeutic utility to prevent spontaneous calcium release-induced atrial electrical activity; especially in patients with a history of paroxysmal AF displaying preserved ICa density.
Descripció
Matèries (anglès)
Citació
Citació
JIMÉNEZ SÁBADO, Verónica, CASABELLA-RAMÓN, Sergi, LLACH, Anna, GICH, Ignasi, CASELLAS, Sandra, CIRUELA ALFÉREZ, Francisco, CHEN, S.r. wayne, GUERRA, José maría, GINEL, Antonino, BENÍTEZ, Raul, CINCA, Juan, TARIFA, Carmen, HOVE-MADSEN, Leif. Beta-blocker treatment of patients with atrial fibrillation attenuates abnormal electrical activity induced by spontaneous calcium release. _Biomedicine & Pharmacotherapy_. 2023. Vol. 158. [consulta: 24 de novembre de 2025]. ISSN: 0753-3322. [Disponible a: https://hdl.handle.net/2445/224177]