Current directions in DNA gel particles

Abstract

A general understanding of interactions between DNA and oppositely charged compounds forms the basis for developing novel DNA-based materials, including gel particles. The association strength, which is altered by varying the chemical structure of the cationic cosolute, determines the spatial homogeneity of the gelation process, creating DNA reservoir devices and DNA matrix devices that can be designed to release either single- (ssDNA) or double-stranded (dsDNA) DNA. This paper reviews the preparation of DNA gel particles using surfactants, proteins and polysaccharides. Particle morphology, swelling/dissolution behaviour, degree of DNA entrapment and DNA release responses as a function of the nature of the cationic agent used are discussed. Current directions in the haemocompatible and cytotoxic characterization of these DNA gel particles have been also included.