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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/133610
Endophilin drives the fast mode of vesicle retrieval in a ribbon synapse
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Compensatory endocytosis of exocytosed membrane and recycling of synaptic vesicle components is essential for sustained synaptic transmission at nerve terminals. At the ribbon-type synapse of retinal bipolar cells, manipulations expected to inhibit the interactions of the clathrin adaptor protein complex (AP2) affect only the slow phase of endocytosis (τ = 10-15 s), leading to the conclusion that fast endocytosis (τ = 1-2 s) occurs by a mechanism that differs from the classical pathway of clathrin-coated vesicle retrieval from the plasma membrane. Here we investigate the role of endophilin in endocytosis at this ribbon synapse. Endophilin A1 is a synaptically enriched N-BAR domain-containing protein, suggested to function in clathrin-mediated endocytosis. Internal dialysis of the synaptic terminal with dominant-negative endophilin A1 lacking its linker and Src homology 3 (SH3) domain inhibited the fast mode of endocytosis, while slow endocytosis continued. Dialysis of a peptide that binds endophilin SH3 domain also decreased fast retrieval. Electron microscopy indicated that fast endocytosis occurred by retrieval of small vesicles in most instances. These results indicate that endophilin is involved in fast retrieval of synaptic vesicles occurring by a mechanism that can be distinguished from the classical pathway involving clathrin-AP2 interactions.
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LLOBET BERENGUER, Artur, GALLOP, Jennifer l., BURDEN, Jemima, ÇAMDERE, Gamze, CHANDRA, Priya, VALLIS, Yvonne, HOPKINS, Colin r., LAGNADO, Leon, MCMAHON, Harvey t.. Endophilin drives the fast mode of vesicle retrieval in a ribbon synapse. _Journal of Neuroscience_. 2011. Vol. 31, núm. 23, pàgs. 8512-8519. [consulta: 23 de gener de 2026]. ISSN: 0270-6474. [Disponible a: https://hdl.handle.net/2445/133610]