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cc by (c) Rocha, Ana Sofia et al, 2023
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/204384

Luminal Rank loss decreases cell fitness leading to basal cell bipotency in parous mammary glands

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Rank signaling pathway regulates mammary gland homeostasis and epithelial cell differentiation. Although Rank receptor is expressed by basal cells and luminal progenitors, its role in each individual cell lineage remains unclear. By combining temporal/lineage specific Rank genetic deletion with lineage tracing techniques, we found that loss of luminal Rank reduces the luminal progenitor pool and leads to aberrant alveolar-like differentiation with high protein translation capacity in virgin mammary glands. These Rank-deleted luminal cells are unable to expand during the first pregnancy, leading to lactation failure and impairment of protein synthesis potential in the parous stage. The unfit parous Rank-deleted luminal cells in the alveoli are progressively replaced by Rank-proficient cells early during the second pregnancy, thereby restoring lactation. Transcriptomic analysis and functional assays point to the awakening of basal bipotency after pregnancy by the induction of Rank/NF-kappa B signaling in basal parous cell to restore lactation and tissue homeostasis. Rocha and co-authors show that loss of luminal Rank signaling causes abnormal alveolar differentiation and lactation failure. Subsequent pregnancies activate bipotency in basal cells, replacing unfit luminal cells, and restoring lactation.

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ROCHA, Ana sofia, COLLADO SOLÉ, Alejandro, GRAÑA CASTRO, Osvaldo, REDONDO PEDRAZA, Jaime, SORIA ALCAIDE, Gonzalo, CORDERO, Alex, SANTAMARÍA, Patricia g., GONZÁLEZ SUÁREZ, Eva. Luminal Rank loss decreases cell fitness leading to basal cell bipotency in parous mammary glands. _Nature Communications_. 2023. Vol. 14, núm. 1. [consulta: 24 de gener de 2026]. ISSN: 2041-1723. [Disponible a: https://hdl.handle.net/2445/204384]

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