Files
Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/194080
The absence of FAIM leads to a delay in dark adaptation and hampers arrestin-1 translocation upon light reception in the retina
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
The short and long isoforms of FAIM (FAIM-S and FAIM-L) hold important functions in the central nervous system, and their expression levels are specifically enriched in the retina. We previously described that Faim knockout (KO) mice present structural and molecular alterations in the retina compatible with a neurodegenerative phenotype. Here, we aimed to study Faim KO retinal functions and molecular mechanisms leading to its alterations. Electroretinographic recordings showed that aged Faim KO mice present functional loss of rod photoreceptor and ganglion cells. Additionally, we found a significant delay in dark adaptation from early adult ages. This functional deficit is exacerbated by luminic stress, which also caused histopathological alterations. Interestingly, Faim KO mice present abnormal Arrestin-1 redistribution upon light reception, and we show that Arrestin-1 is ubiquitinated, a process that is abrogated by either FAIM-S or FAIM-L in vitro. Our results suggest that FAIM assists Arrestin-1 light-dependent translocation by a process that likely involves ubiquitination. In the absence of FAIM, this impairment could be the cause of dark adaptation delay and increased light sensitivity. Multiple retinal diseases are linked to deficits in photoresponse termination, and hence, investigating the role of FAIM could shed light onto the underlying mechanisms of their pathophysiology.
Subject (English)
Citation
Citation
SIRÉS, Anna, et al. The absence of FAIM leads to a delay in dark adaptation and hampers arrestin-1 translocation upon light reception in the retina. Cells. 2023. Vol. 12, num. 3, pags. 487. ISSN 2073-4409. [consulted: 10 of August of 2026]. Available at: https://hdl.handle.net/2445/194080