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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/215752
Effects of intermittent exposure to hypobaric hypoxia and cold on skeletal muscle regeneration: mitochondrial dynamics, protein oxidation and turnover
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Muscle injuries and the subsequent regeneration events compromise muscle homeostasis at morphological, functional and molecular levels. Among the molecular alterations, those derived from the mitochondrial function are especially relevant. We analysed the mitochondrial dynamics, the redox balance, the protein oxidation and the main protein repairing mechanisms after 9 days of injury in the rat gastrocnemius muscle. During the recovery rats were exposed to intermittent cold exposure (ICE), intermittent hypobaric hypoxia (IHH), and both simultaneous combined stimuli. Non-injured contralateral legs were also analysed to evaluate the specific effects of the three environmental exposures. Our results showed that ICE enhanced mitochondrial adaptation by improving the electron transport chain efficiency during muscle recovery, decreased the expression of regulatory subunit of proteasome and accumulated oxidised proteins. Exposure to IHH did not show mitochondrial compensation or increased protein turnover mechanisms; however, no accumulation of oxidized proteins was observed. Both ICE and IHH, when applied separately, elicited an increased expression of eNOS, which could have played an important role in accelerating muscle recovery. The combined effect of ICE and IHH led to a complex response that could potentially impede optimal mitochondrial function and enhanced the accumulation of protein oxidation. These findings underscore the nuanced role of environmental stressors in the muscle healing process and their implications for optimizing recovery strategies.
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SÁNCHEZ NUÑO, Sergio, et al. Effects of intermittent exposure to hypobaric hypoxia and cold on skeletal muscle regeneration: mitochondrial dynamics, protein oxidation and turnover. Free Radical Biology and Medicine. 2024. Vol. 225, num. 286-295. ISSN 0891-5849. [consulted: 11 of October of 2026]. Available at: https://hdl.handle.net/2445/215752