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2011-12-01

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cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/52756

A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus

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http://dx.doi.org/10.2436/20.1501.01.149

Abstract

Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.

Subject

Fermentació, Saccharomyces cerevisiae, Genòmica, Vi

Subject (English)

Fermentation, Saccharomyces cerevisiae, Genomics, Wine

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Articles publicats en revistes (Patologia i Terapèutica Experimental)

Citation

BLASCO, Lucía, et al. A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus. International Microbiology. 2011. Vol. 14, num. 201-206. ISSN 1139-6709. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/52756

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