A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus

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dc.contributor.authorBlasco, Lucía
dc.contributor.authorVeiga Crespo, Patricia
dc.contributor.authorViñas, Miquel
dc.contributor.authorVilla, Tomás G.
dc.date.accessioned2014-03-21T13:18:31Z
dc.date.available2014-03-21T13:18:31Z
dc.date.issued2011-12-01
dc.date.updated2014-03-21T13:18:31Z
dc.description.abstractYeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.
dc.format.extent6 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idgrec614979
dc.identifier.issn1139-6709
dc.identifier.urihttps://hdl.handle.net/2445/52756
dc.language.isoeng
dc.publisherSpanish Society for Microbiology (SEM) and Viguera Editores SL
dc.relation.isformatofReproducció del document publicat a: http://dx.doi.org/10.2436/20.1501.01.149
dc.relation.ispartofInternational Microbiology, 2011, vol. 14, p. 201-206
dc.relation.urihttp://dx.doi.org/10.2436/20.1501.01.149
dc.rightscc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/es
dc.sourceArticles publicats en revistes (Patologia i Terapèutica Experimental)
dc.subject.classificationFermentació
dc.subject.classificationSaccharomyces cerevisiae
dc.subject.classificationGenòmica
dc.subject.classificationVi
dc.subject.otherFermentation
dc.subject.otherSaccharomyces cerevisiae
dc.subject.otherGenomics
dc.subject.otherWine
dc.titleA new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion

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