Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/126893
The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Epithelial morphogenesis and stability are essential for normal development and organ homeostasis. The mouse neural plate is a cuboidal epithelium that remodels into a columnar pseudostratified epithelium over the course of 24 hr. Here we show that the transition to a columnar epithelium fails in mutant embryos that lack the tumor suppressor PTEN, although proliferation, patterning and apical-basal polarity markers are normal in the mutants. The Pten phenotype is mimicked by constitutive activation of P13 kinase and is rescued by the removal of PDK1 (PDPK1), but does not depend on the downstream kinases AKT and mTORC1. High resolution imaging shows that PTEN is required for stabilization of planar cell packing in the neural plate and for the formation of stable apical-basal microtubule arrays. The data suggest that appropriate levels of membrane-associated PDPK1 are required for stabilization of apical junctions, which promotes cell elongation, during epithelial morphogenesis.
Matèries
Matèries (anglès)
Citació
Citació
GREGO BESSA, Joaquim, BLOOMEKATZ, Joshua, CASTEL MORALES, Pau, OMELCHENKO, Tatiana, BASELGA TORRES, Josep, ANDERSON, Kathryn v.. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. _Elife_. 2016. Vol. 5, núm. e12034. [consulta: 7 de febrer de 2026]. [Disponible a: https://hdl.handle.net/2445/126893]