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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/182834
Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum
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--- - i: - Plasmodium falciparum content: - "Malaria transmission requires that some asexual parasites convert into sexual forms termed gametocytes. The initial stages of sexual development, including sexually committed schizonts and sexual rings, remain poorly haracterized, mainly because they are morphologically identical to their sexual counterparts and only a small subset of parasites undergo sexual development. Here, we describe a system for controlled sexual conversion in the human
malaria parasite " - ", based on conditional expression of the
PfAP2-G transcription factor. Using this system, ~90 percent of
the parasites converted into sexual forms upon induction,
enabling the characterization of committed and early sexual
stages without further purification. We characterized sexually
committed schizonts and sexual rings at the transcriptomic and
phenotypic levels, which revealed down-regulation of genes
involved in solute transport upon sexual commitment, among other
findings. The new inducible lines will facilitate the study of
early sexual stages at additional levels, including multiomic
characterization and drug susceptibility assays."
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LLORÀ BATLLE, Oriol, MICHEL TODÓ, Lucas, WITMER, Kathrin, TODA, Haruka, FERNÁNDEZ BECERRA, Carmen, BAUM, Jake, CORTÉS, Alfred. Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum. _Science advances_. 2020. Vol. vol 6, núm. num 24. [consulta: 27 de gener de 2026]. ISSN: 2375-2548. [Disponible a: https://hdl.handle.net/2445/182834]