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cc-by (c) Puig, Pere et al., 2019
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/164367

Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer

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Objectives: To apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. Results: The systems pathology approach applied allows a precise quantification of the marker expression in the different cell compartments as well as the determination of the areas that coexpress two markers. Our data shows that the accumulation of β-catenin in the nuclear compartment is significantly decreased in the adjacent normal areas when compared to tumor of the same patients (p < 0.001). In conclusion, the application of this novel multiple immunofluorescence assay demonstrates that the upregulation of Active β-catenin is a relatively common feature of prostate tumor development, and further supports the activation of the Wnt/β-catenin pathway in prostate cancer progression.

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PUIG, Pere, ERILL, Nadina, TERRICABRAS, Marta, SUBIRANA, Isaac, GONZÁLEZ-GARCÍA, Judit, ASENSI-PUIG, Adrià, DONOVAN, Michael j., MENGUAL BRICHS, Lourdes, AGULLÓ-ORTUÑO, M. teresa, OLIVAN RIERA, Mireia, ALCARAZ ASENSIO, Antonio, LÓPEZ-MARTÍN, José a., TORRES, Inés de, RODRÍGUEZ PERALTO, José luis, RODRÍGUEZ-ANTOLÍN, Alfredo, MOROTE, Juan, GONZÁLEZ-RUMAYOR, Víctor. Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer. _BMC Research Notes_. 2019. Vol. 12, núm. 68. [consulta: 27 de gener de 2026]. ISSN: 1756-0500. [Disponible a: https://hdl.handle.net/2445/164367]

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