Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

663353.pdf (724.69 KB)

Document type

Article

Version

Accepted version

Publication date

2016-10-01

All rights reserved

Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/132807

Use of metabolomics and lipidomics to evaluate the hypocholestreolemic effect of Proanthocyanidins from grape seed in a pig model.

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.1002/mnfr.201600190

Abstract

Scope:This work aims to evaluate changes in the fecal metabolomic profile due to grapeseed extract (GSE) intake by untargeted and targeted analysis using high resolution massspectrometry in conjunction with multivariate statistics.Methods and results:An intervention study with six crossbred female pigs was performed. Thepigs followed a standard diet for 3 days, then they were fed with a supplemented diet containing1% (w/w) of MegaNaturalR©Gold grape seed extract for 6 days. Fresh pig fecal samples werecollected daily. A combination of untargeted high resolution mass spectrometry, multivariateanalysis (PLS-DA), data-dependent MS/MS scan, and accurate mass database matching wasused to measure the effect of the treatment on fecal composition. The resultant PLS-DAmodels showed a good discrimination among classes with great robustness and predictability.A total of 14 metabolites related to the GSE consumption were identified including biliary acid,dicarboxylic fatty acid, cholesterol metabolites, purine metabolites, and eicosanoid metabolitesamong others. Moreover, targeted metabolomics using GC-MS showed that cholesterol andits metabolites fecal excretion was increased due to the proanthocyanidins from grape seedextract.Conclusion:The results show that oligomeric procyanidins from GSE modifies bile acid andsteroid excretion, which could exert a hypocholesterolemic effect.

Subject

Metabolisme, Botànica, Llavors, Raïms, Experimentació animal, Antioxidants, Colesterol, Espectroscòpia

Subject (English)

Metabolism, Botany, Seeds, Grapes, Animal experimentation, Antioxidants, Cholesterol, Spectrum analysis

Citation

Collections

Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

Citation

QUIFER RADA, Paola, et al. Use of metabolomics and lipidomics to evaluate the hypocholestreolemic effect of Proanthocyanidins from grape seed in a pig model. Molecular Nutrition & Food Research. 2016. Vol. 60, pags. 2219-2227. ISSN 1613-4125. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/132807

Export metadata

JSON - METS

Share record