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1994

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/8302

Expression cloning of a rat hepatic reduced glutathione transporter with canalicular characteristics

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http://dx.doi.org/10.1172/JCI117170

Resum

Using the Xenopus oocyte expression system, we have previously identified an approximately 4-kb fraction of mRNA from rat liver that expresses sulfobromophthalein-glutathione (BSP-GSH)-insensitive reduced glutathione (GSH) transport (Fernandez-Checa, J., J. R. Yi, C. Garcia-Ruiz, Z. Knezic, S. Tahara, and N. Kaplowitz. 1993. J. Biol. Chem. 268:2324-2328). Starting with a cDNA library constructed from this fraction, we have now isolated a single clone that expresses GSH transporter activity. The cDNA for the rat canalicular GSH transporter (RcGshT) is 4.05 kb with an open reading frame of 2,505 nucleotides encoding for a polypeptide of 835 amino acids (95,785 daltons). No identifiable homologies were found in searching various databases. An approximately 96-kD protein is generated in in vitro translation of cRNA for RcGshT. Northern blot analysis reveals a single 4-kb transcript in liver, kidney, intestine, lung, and brain. The abundance of mRNA for RcGshT in rat liver increased 3, 6, and 12 h after a single dose of phenobarbital. Insensitivity to BSP-GSH and induction by phenobarbital, unique characteristics of canalicular GSH secretion, suggest that RcGshT encodes for the canalicular GSH transporter.

Matèries

Glutatió, Fetge, Metabolisme, Proteïnes de membrana, Transport biològic, Clonatge

Matèries (anglès)

Glutathione, Liver, Membrane proteins, Metabolism, Cloning

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Articles publicats en revistes (Medicina)

Citació

YI, Jian-ri, LU, Shilun, FERNÁNDEZ-CHECA TORRES, José carlos, KAPLOWITZ, Neil. Expression cloning of a rat hepatic reduced glutathione transporter with canalicular characteristics. _Journal of Clinical Investigation_. 1994. Vol. 93, núm. 4, pàgs. 1841-1845. [consulta: 20 de gener de 2026]. ISSN: 0021-9738. [Disponible a: https://hdl.handle.net/2445/8302]

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