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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/181798
Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
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Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance.
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Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB))
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB))
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
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COLOMÉ, Núria, ABIÁN, Joaquín, ALORIA, Kerman, ARIZMENDI, Jesús m., BARCELÓ BATLLORI, Sílvia, BRAGA LAGACHE, Sophie, BURLET SCHILTZ, Odile, CARRASCAL, Montse, CASAL, J. ignacio, CHICANO GÁLVEZ, Eduard, CHIVA, Cristina, CLEMENTE, Luis felipe, ELORTZA, Felix, ESTANYOL I ULLATE, Josep maria, FERNANDEZ IRIGOYEN, Joaquín, FERNÁNDEZ PUENTE, Patricia, FIDALGO, María josé, FROMENT, Carine, FUENTES, Manuel, FUENTES ALMAGRO, Carlos, GAY, Marina, HAINARD, Alexandre, HELLER, Manfred, HERNÁNDEZ, María luisa, IBARROLA, Nieves, ILORO, Ibon, KIESELBACH, Thomas, LARIO, Antonio, LOCARD PAULET, Marie, MARINA RAMÍREZ, Anabel, MARTÍN, Luna, MORATO LÓPEZ, Esperanza, MUÑOZ, Javier, NAVAJAS, Rosana, ODENA, M. antonia, ODRIOZOLA, Leticia, OLIVEIRA, Eliandre, PARADELA, Alberto, PASQUARELLO MOSIMANN, Carla, RIOS, Vivian de los, RUIZ ROMERO, Cristina, SABIDÓ AGUADÉ, Eduard, SÁNCHEZ DEL PINO, Manuel, SANCHO, Jaime, SANTAMARÍA, Enrique, SCHAEFFER REISS, Christine, SCHNEIDER, Justine, TORRE, Carolina de la, VALERO, M. luz, VILASECA, Marta, WU, Shuai, WU, Linfeng, XIMÉNEZ EMBÚN, Pilar, CANALS, Francesc, CORRALES, Fernando. Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis. _Journal of Proteomics_. 2021. Vol. 251. [consulta: 27 de novembre de 2025]. ISSN: 1874-3919. [Disponible a: https://hdl.handle.net/2445/181798]