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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/68708
In vivo and in vitro characterization of a Plasmodium liver stage-specific promoter
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Abstract
Little is known about stage-specific gene regulation in
Plasmodium parasites, in particular the liver stage of
development. We have previously described in the Plasmodium
berghei rodent model, a liver stage-specific (lisp2) gene
promoter region, in vitro. Using a dual luminescence system, we
now confirm the stage specificity of this promoter region also
in vivo. Furthermore, by substitution and deletion analyses we
have extended our in vitro characterization of important
elements within the promoter region. Importantly, the dual
luminescence system allows analyzing promoter constructs
avoiding mouse-consuming cloning procedures of transgenic
parasites. This makes extensive mutation and deletion studies a
reasonable approach also in the malaria mouse model.
Stage-specific expression constructs and parasite lines are
extremely valuable tools for research on Plasmodium liver stage
biology. Such reporter lines offer a promising opportunity for
assessment of liver stage drugs, characterization of genetically
attenuated parasites and liver stage-specific vaccines both in
vivo and in vitro, and may be key for the generation of
inducible systems.
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NIZ, Mariana De, et al. In vivo and in vitro characterization of a Plasmodium liver
stage-specific promoter. PLoS One. 2015. Vol. 10, num. 4, pags. e0123473. ISSN 1932-6203. [consulted: 8 of June of 2026]. Available at: https://hdl.handle.net/2445/68708