Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/123876
Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The contamination of adenovirus (Ad) stocks with adeno-associated viruses (AAV) is usually unnoticed, and it has been associated with lower Ad yields upon large-scale production. During Ad propagation, AAV contamination needs to be detected routinely by polymerase chain reaction without symptomatic suspicion. In this study, we describe that the coinfection of either Ad wild type 5 or oncolytic Ad with AAV results in a large-plaque phenotype associated with an accelerated release of Ad from coinfected cells. This accelerated release was accompanied with the expected decrease in Ad yields in two out of three cell lines tested. Despite this lower Ad yield, coinfection with AAV accelerated cell death and enhanced the cytotoxicity mediated by Ad propagation. Intratumoral coinjection of Ad and AAV in two xenograft tumor models improved antitumor activity and mouse survival. Therefore, we conclude that accidental or intentional AAV coinfection has important implications for Ad-mediated virotherapy.
Matèries
Matèries (anglès)
Citació
Citació
LABORDA, Eduardo, PUIG SAUS, Cristina, CASCALLÒ, Manel, CHILLÓN, Miguel, ALEMANY BONASTRE, Ramon. Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread. _Human Gene Therapy Methods_. 2013. Vol. 24, núm. 6, pàgs. 372-380. [consulta: 14 de gener de 2026]. [Disponible a: https://hdl.handle.net/2445/123876]