Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

Bio-protocol1120.pdf (440.08 KB)

Document type

Article

Version

Published version

Publication date

2014-03-05

All rights reserved

Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/183467

Immunostaining Protocol: P-Stat3 (Xenograft and Mice)

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.21769/BioProtoc.1120

Abstract

We sought to understand the mechanisms behind the potent effect of stromal TGF-beta program on the capacity of colorectal cancer (CRC) cells to initiate metastasis. We discovered that mice subcutaneous tumors and metastases generated in the context of a TGF-beta activated microenvironment displayed prominent accumulation of p-STAT3 in CRC cells compared with those derived from control cells. STAT3 signaling depended on GP130 as shown by strong reduction of epithelial p STAT3 levels upon GP130 shRNA-mediated knockdown in CRC cells.

Subject

Càncer colorectal, Metàstasi, Expressió gènica

Subject (English)

Colorectal cancer, Metastasis, Gene expression

Citation

Collections

Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Citation

CALON, Alexandre, et al. Immunostaining Protocol: P-Stat3 (Xenograft and Mice). Bio-protocol. 2014. Vol. 4, num. 9, pags. e1120. ISSN 2331-8325. [consulted: 10 of August of 2026]. Available at: https://hdl.handle.net/2445/183467

Export metadata

JSON - METS

Share record