Miniatura

Fitxers

Moreno_2018.pdf (895.53 KB)

Tipus de document

Article

Versió

Versió acceptada

Data de publicació

2018-06-14

Llicència de publicació

cc by-nc-nd (c) Elsevier, 2018
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/124007

Telomere length alterations in microsatellite stable colorectal cancer and association with the immune response

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1016/j.bbadis.2018.06.010

Resum

Telomeres are repetitive sequences (TTAGGG) located at the end of chromosomes. Telomeres progressively shorten with each cell replication cycle, ultimately leading to chromosomal instability and loss of cell viability. Telomere length anomaly appears to be one of the earliest and most prevalent genetic alterations in malignant transformation. Here we aim to estimate telomere length from whole-exome sequencing data in colon tumors and normal colonic mucosa, and to analyze the potential association of telomere length with clinical factors and gene expression in colon cancer. Reads containing at least five repetitions of the telomere sequence (TTAGGG) were extracted from the raw sequences of 42 adjacent normal-tumor paired samples. The number of reads from the tumor sample was normalized to build the Tumor Telomere Length Ratio (TTLR), considered an estimation of telomere length change in the tumor compared to the paired normal tissue. We evaluated the associations between TTLR and clinical factors, gene expression and copy number (CN) aberrations measured in the same tumor samples. Colon tumors showed significantly shorter telomeres than their paired normal samples. No significant association was observed between TTLR and gender, age, tumor location, prognosis, stromal infiltration or molecular subtypes. The functional gene set enrichment analysis showed pathways related to immune response significantly associated with TLLR. By extracting a relative measure of telomere length from whole-exome sequencing data, we have assessed that colon tumor cells predominantly shorten telomeres, and this alteration is associated with expression changes in genes related to immune response and inflammation in tumor cells.

Matèries

Telòmer, Càncer colorectal

Matèries (anglès)

Telomere, Colorectal cancer

Citació

Col·leccions

Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

LÓPEZ DÓRIGA GUERRA, Adriana, SANZ PAMPLONA, Rebeca, VALLE VELASCO, Laura, ALONSO AGUADO, Maria henar, AUSSÓ, Susanna, CLOSA, Adrià, SANJUAN, Xavier, BARQUERO, David, RODRÍGUEZ MORANTA, Francisco, MORENO AGUADO, Víctor. Telomere length alterations in microsatellite stable colorectal cancer and association with the immune response. _Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease_. 2018. Vol. 1864, núm. 9 (part B), pàgs. 2992-3000. [consulta: 28 de gener de 2026]. ISSN: 0925-4439. [Disponible a: https://hdl.handle.net/2445/124007]

Exportar metadades

JSON - METS

Compartir registre