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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/202625
Combination of protein and cell internalization SELEX identifies a potential RNA therapeutic and delivery platform to treat EphA2-expressing tumors
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The EphA2 receptor tyrosine kinase is overexpressed in most solid tumors and acts as the major driver of tumorigenesis. In this study, we developed a novel approach for targeting the EphA2 receptor using a 20-fluoro-modified pyrimidine RNA aptamer termed ATOP. We identified the ATOP EphA2 aptamer using a novel bioinformatics strategy that compared aptamers enriched during a protein SELEX using recombinant human EphA2 and a cell-internalization SELEX using EphA2-expressing MDA231 tumor cells. When applied to EphA2-expressing tumor cell lines, the ATOP EphA2 aptamer attenuated tumor cell migration and clonogenicity. In a mouse model of spontaneous metastasis, the ATOP EphA2 aptamer slowed primary tumor growth and significantly reduced the number of lung metastases. The EphA2 ATOP aptamer represents a promising candidate for the development of next-generation targeted therapies that provide safer and more effective treatment of EphA2-overexpressing tumors.
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SANTANA VIERA, Laura, DASSIE, Justin p., ROSÀS LAPEÑA, Marta, GARCIA MONCLÚS, Silvia, CHICÓN BOSCH, Mariona, PÉREZ CAPÓ, Marina, POZO, Lidia del, SÁNCHEZ SERRA, Sara, ALMACELLAS RABAIGET, Olga, MAQUEDA MARCOS, Susana, LÓPEZ ALEMANY, Roser, THIEL, William h., GIANGRANDE, Paloma h., TIRADO, Oscar m.. Combination of protein and cell internalization SELEX identifies a potential RNA therapeutic and delivery platform to treat EphA2-expressing tumors. _Molecular Therapy - Nucleic Acids_. 2023. Vol. 32, núm. 758-772. [consulta: 26 de febrer de 2026]. ISSN: 2162-2531. [Disponible a: https://hdl.handle.net/2445/202625]