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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/229286
Optimization of agro-technological processing parameters to enhance diamine oxidase activity in edible legume sprouts
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Histamine intolerance is associated with impaired histamine metabolism caused by a deficiency of the enzyme diamine oxidase (DAO). A recommended strategy to alleviate symptoms is a low-histamine diet supplemented with exogenous DAO. Recent studies suggest plant-based DAO from legume sprouts could be a viable active ingredient for supplements. This study aimed to assess the impact of germination parameters (time-temperature binomial and salinity stress) on the histamine-degrading enzymatic activity of four species of edible Leguminosae sprouts and its stability during different storage conditions. The time-temperature binomial was found to strongly influence sprout DAO activity. Chickpea sprouts exhibited the highest activity at 14 °C on day 8 of germination, whereas lentil, soybean, and green pea sprouts showed peak activity at 30 °C between days 4 and 5. On the other hand, salinity stress did not enhance DAO activity and reduced the germination rate by 20–30 % compared to the control. Freezing was the only storage condition that preserved the DAO activity of the active ingredient intact for at least 12 months.
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COSTA-CATALA, Judit, et al. Optimization of agro-technological processing parameters to enhance diamine oxidase activity in edible legume sprouts. Journal of Agriculture and Food Research. 2025. Vol. 22. [consulted: 19 of August of 2026]. Available at: https://hdl.handle.net/2445/229286