Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/184454
Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue's extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher(R) S microcarriers' (MCs') as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.
Subject (English)
Citation
Citation
RUBI SANS, Gerard, et al. Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template. Biomedicines. 2021. Vol. vol 9, num. num 3. ISSN 2227-9059. [consulted: 10 of August of 2026]. Available at: https://hdl.handle.net/2445/184454