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2014-01-01

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/226001

Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants

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https://doi.org/10.1007/978-1-4939-0606-2_3

Resum

The enzyme 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase catalyzes the NADPH-mediated reductive deacylation of HMG-CoA to mevalonic acid, which is the first committed step of the mevalonate pathway for isoprenoid biosynthesis. In agreement with its key regulatory role in the pathway, plant HMG-CoA reductase is modulated by many diverse external stimuli and endogenous factors and can be detected to variable levels in every plant tissue. A fine determination of HMG-CoA reductase activity levels is required to understand its contribution to plant development and adaptation to changing environmental conditions. Here, we report a procedure to reliably determine HMG-CoA reductase activity in plants. The method includes the sample collection and homogenization strategies as well as the specific activity determination based on a classical radiochemical assay.

Matèries

Fisiologia vegetal, Compostos bioactius vegetals, Metabolisme de les plantes

Matèries (anglès)

Plant physiology, Plant bioactive compounds, Plant metabolism

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

CAMPOS MARTÍNEZ, Narciso, ARRÓ I PLANS, Montserrat, FERRER I PRATS, Albert, BORONAT I MARGOSA, Albert. Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants. _Methods in Molecular Biology_. 2014. Vol. 1153, núm. 21-40. [consulta: 31 de gener de 2026]. ISSN: 1940-6029. [Disponible a: https://hdl.handle.net/2445/226001]

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