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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/226001
Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants
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The enzyme 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase catalyzes the NADPH-mediated reductive deacylation of HMG-CoA to mevalonic acid, which is the first committed step of the mevalonate pathway for isoprenoid biosynthesis. In agreement with its key regulatory role in the pathway, plant HMG-CoA reductase is modulated by many diverse external stimuli and endogenous factors and can be detected to variable levels in every plant tissue. A fine determination of HMG-CoA reductase activity levels is required to understand its contribution to plant development and adaptation to changing environmental conditions. Here, we report a procedure to reliably determine HMG-CoA reductase activity in plants. The method includes the sample collection and homogenization strategies as well as the specific activity determination based on a classical radiochemical assay.
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CAMPOS MARTÍNEZ, Narciso, et al. Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants. Methods in Molecular Biology. 2014. Vol. 1153, num. 21-40. ISSN 1940-6029. [consulted: 13 of June of 2026]. Available at: https://hdl.handle.net/2445/226001