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2018-03-05

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cc by (c) Rubio et al., 2018
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/121074

Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk

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http://dx.doi.org/10.1371/journal.pone.0193527

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Circulating small RNAs, including miRNAs but also isomiRs and other RNA species, have the potential to be used as non-invasive biomarkers for communicable and non-communicable diseases. This study aims to characterize and compare small RNA profiles in human biofluids. For this purpose, RNA was extracted from plasma and breast milk samples from 15 healthy postpartum mothers. Small RNA libraries were prepared with the NEBNext(R) small RNA library preparation kit and sequenced in an Illumina HiSeq2000 platform. miRNAs, isomiRs and clusters of small RNAs were annotated using seqBuster/seqCluster framework in 5 plasma and 10 milk samples that passed the initial quality control. The RNA yield was 81 ng/mL [standard deviation (SD): 41] and 3985 ng/mL (SD: 3767) for plasma and breast milk, respectively. Mean number of good quality reads was 4.04 million (M) (40.01% of the reads) in plasma and 12.5M (89.6%) in breast milk. One thousand one hundred eighty two miRNAs, 12,084 isomiRs and 1,053 small RNA clusters that included piwi-interfering RNAs (piRNAs), tRNAs, small nucleolar RNAs (snoRNA) and small nuclear RNAs (snRNAs) were detected. Samples grouped by biofluid, with 308 miRNAs, 1,790 isomiRs and 778 small RNA clusters differentially detected. In summary, plasma and milk showed a different small RNA profile. In both, miRNAs, piRNAs, tRNAs, snRNAs, and snoRNAs were identified, confirming the presence of non-miRNA species in plasma, and describing them for the first time in milk.

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Micro RNAs, Llet materna

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MicroRNAs, Breast milk

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RUBIO, Mercedes, BUSTAMANTE PINEDA, Mariona, HERNÁNDEZ FERRER, Carles, FERNÁNDEZ ORTH, Dietmar, PANTANO, Lorena, SARRIA TRUJILLO, Yaris, PIQUÉ BORRAS, Maria, VELLVÉ, Kilian, AGRAMUNT, Silvia, CARRERAS, Ramon, ESTIVILL, Xavier, GONZÁLEZ, Juan ramón, MAYOR APARICIO, Alfredo gabriel. Circulating miRNAs, isomiRs and small RNA clusters in human
                plasma and breast milk. _PLoS One_. 2018. Vol. 13, núm. 3, pàgs. e0193527. [consulta: 21 de gener de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/121074]

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