El CRAI romandrà tancat del 24 de desembre de 2025 al 6 de gener de 2026. La validació de documents es reprendrà a partir del 7 de gener de 2026.
El CRAI permanecerá cerrado del 24 de diciembre de 2025 al 6 de enero de 2026. La validación de documentos se reanudará a partir del 7 de enero de 2026.
From 2025-12-24 to 2026-01-06, the CRAI remain closed and the documents will be validated from 2026-01-07.
 
Miniatura

Fitxers

648018.pdf (764.22 KB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2015-03-05

Llicència de publicació

cc-by (c) Arriarán, Sofía et al., 2015
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/65533

Evidences of basal lactate production in the main white adipose tissue sites of rats. Effects of sex and a cafeteria diet. 

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

http://dx.doi.org/10.1371/journal.pone.0119572

Resum

Female and male adult Wistar rats were fed standard chow or a simplified cafeteria diet for one month. Then, the rats were killed and the white adipose tissue (WAT) in four sites: perigonadal, retroperitoneal, mesenteric and subcutaneous (inguinal) were sampled and frozen. The complete WAT weight in each site was measured. Gene expression analysis of key lipid and glucose metabolism enzymes were analyzed, as well as tissue and plasma lactate and the activity of lactate dehydrogenase. Lactate gradients between WAT and plasma were estimated. The influence of sex and diet (and indirectly WAT mass) on lactate levels and their relationships with lactate dehydrogenase activity and gene expressions were also measured. A main conclusion is the high production of lactate by WAT, practically irrespective of site, diet or sex. Lactate production is a direct correlate of lactate dehydrogenase activity in the tissue. Furthermore, lactate dehydrogenase activity is again directly correlated with the expression of the genes Ldha and Ldhb for this enzyme. In sum, the ability to produce lactate by WAT is not directly dependent of WAT metabolic state.We postulate that, in WAT, a main function of the lactate dehydrogenase path may be that of converting excess available glucose to 3C fragments, as a way to limit tissue self-utilization as substrate, to help control glycaemia and/or providing short chain substrates for use as energy source elsewhere. More information must be gathered before a conclusive role of WAT in the control of glycaemia, and the full existence of a renewed glucose-lactate-fatty acid cycle is definitely established.

Matèries

Teixit adipós, Hidroxiàcids, Metabolisme, Dieta, Expressió gènica, Diferències entre sexes, Rates (Animals de laboratori)

Matèries (anglès)

Adipose tissues, Hydroxy acids, Metabolism, Diet, Gene expression, Sex differences, Rats as laboratory animals

Citació

Col·leccions

Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

Citació

ARRIARÁN, Sofía, AGNELLI, Silvia, SABATER MARTÍNEZ, David, REMESAR BETLLOCH, Xavier, FERNÁNDEZ LÓPEZ, José antonio, ALEMANY, Marià. Evidences of basal lactate production in the main white adipose tissue sites of rats. Effects of sex and a cafeteria diet. . _PLoS One_. 2015. Vol. 10, núm. 3, pàgs. e0119572. [consulta: 7 de gener de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/65533]

Exportar metadades

JSON - METS

Compartir registre