Files
Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/174737
Internalization and viability studies of suspended nanowire silicon chips in HeLa Cells
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
Micrometer-sized silicon chips have been demonstrated to be cell-internalizable, offering the possibility of introducing in cells even smaller nanoelements for intracellular applications. On the other hand, silicon nanowires on extracellular devices have been widely studied as biosensors or drug delivery systems. Here, we propose the integration of silicon nanowires on cell-internalizable chips in order to combine the functional features of both approaches for advanced intracellular applications. As an initial fundamental study, the cellular uptake in HeLa cells of silicon 3 m 3 m nanowire-based chips with two different morphologies was investigated, and the results were compared with those of non-nanostructured silicon chips. Chip internalization without affecting cell viability was achieved in all cases; however, important cell behavior differences were observed. In particular, the first stage of cell internalization was favored by silicon nanowire interfaces with respect to bulk silicon. In addition, chips were found inside membrane vesicles, and some nanowires seemed to penetrate the cytosol, which opens the door to the development of silicon nanowire chips as future intracellular sensors and drug delivery systems.
Subject
Subject (English)
Citation
Citation
DURÁN, Sara, et al. Internalization and viability studies of suspended nanowire silicon chips in HeLa Cells. Nanomaterials. 2020. Vol. 10(5), num. 893. ISSN 2079-4991. [consulted: 9 of August of 2026]. Available at: https://hdl.handle.net/2445/174737