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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/171333
Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration
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Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. Our understanding of the cellular and molecular bases that underlie this process, although fragmentary, has increased significantly over the last years. However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. Here, we set out to characterize the ECM protein composition in adult zebrafish hearts, and whether it changed during the regenerative response. For this purpose, we first established a decellularization protocol of adult zebrafish ventricles that significantly enriched the yield of ECM proteins. We then performed proteomic analyses of decellularized control hearts and at different times of regeneration. Our results show a dynamic change in ECM protein composition, most evident at the earliest (7 days postamputation) time point analyzed. Regeneration associated with sharp increases in specific ECM proteins, and with an overall decrease in collagens and cytoskeletal proteins. We finally tested by atomic force microscopy that the changes in ECM composition translated to decreased ECM stiffness. Our cumulative results identify changes in the protein composition and mechanical properties of the zebrafish heart ECM during regeneration.
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GARCIA PUIG, Anna, et al. Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration. Molecular & Cellular Proteomics. 2019. Vol. 18, num. 9, pags. 1745-1755. ISSN 1535-9476. [consulted: 16 of October of 2026]. Available at: https://hdl.handle.net/2445/171333