Miniatura

Fitxers

697533.pdf (739.03 KB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2019-07-23

Llicència de publicació

cc-by (c) Fernández Santiago, Rubén et al., 2019
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/171541

Whole-genome DNA hyper-methylation in iPSC-derived dopaminergic neurons from Parkinson's disease patients

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1186/s13148-019-0701-6

Resum

Background: Parkinson's disease (PD) is characterized by the loss of midbrain dopaminergic neurons (DAn). Previously, we described the presence of DNA hyper- and hypo-methylation alterations in induced pluripotent stem cells (iPSC)-derived DAn from PD patients using the Illumina 450K array which prominently covers gene regulatory regions. Methods: To expand and contextualize previous findings, we performed the first whole-genome DNA bisulfite sequencing (WGBS) using iPSC-derived DAn from representative PD subjects: one sporadic PD (sPD) patient, one monogenic LRRK2-associated PD patient (L2PD), and one control. Results: At the whole-genome level, we detected global DNA hyper-methylation in the PD which was similarly spread across the genome in both sPD and L2PD and mostly affected intergenic regions. Conclusion: This study implements previous epigenetic knowledge in PD at a whole genome level providing the first comprehensive and unbiased CpG DNA methylation data using iPSC-derived DAn from PD patients. Our results indicate that DAn from monogenic or sporadic PD exhibit global DNA hyper-methylation changes. Findings from this exploratory study are to be validated in further studies analyzing other PD cell models and patient tissues.

Matèries

Malaltia de Parkinson, ADN, Metilació

Matèries (anglès)

Parkinson's disease, DNA, Methylation

Citació

Col·leccions

Articles publicats en revistes (Patologia i Terapèutica Experimental)
<b>Publicacions de projectes de recerca finançats per la UE</b>
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Institut de Biomedicina (IBUB))

Citació

FERNÁNDEZ SANTIAGO, Rubén, MERKEL, Angelika, CASTELLANO, Giancarlo, HEATH, Simon, RAYA CHAMORRO, Ángel, TOLOSA, Eduardo, MARTI, Maria jose, CONSIGLIO, Antonella, EZQUERRA TRABALÓN, Mario. Whole-genome DNA hyper-methylation in iPSC-derived dopaminergic neurons from Parkinson's disease patients. _Clinical Epigenetics_. 2019. Vol. 11. [consulta: 20 de gener de 2026]. ISSN: 1868-7075. [Disponible a: https://hdl.handle.net/2445/171541]

Exportar metadades

JSON - METS

Compartir registre