Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/127523
Mycobacteria clumping increase their capacity to damage macrophages
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The rough morphotypes of non-tuberculous mycobacteria have been associated with the most severe illnesses in humans. This idea is consistent with the fact that Mycobacterium tuberculosis presents a stable rough morphotype. Unlike smooth morphotypes, the bacilli of rough morphotypes grow close together, leaving no spaces among them and forming large aggregates (clumps). Currently, the initial interaction of macrophages with clumps remains unclear. Thus, we infected J774 macrophages with bacterial suspensions of rough morphotypes of M. abscessus containing clumps and suspensions of smooth morphotypes, primarily containing isolated bacilli. Using confocal laser scanning microscopy and electron microscopy, we observed clumps of at least five rough-morphotype bacilli inside the phagocytic vesicles of macrophages at 3 h post-infection. These clumps grew within the phagocytic vesicles, killing 100% of the macrophages at 72 h post-infection, whereas the proliferation of macrophages infected with smooth morphotypes remained unaltered at 96 h post-infection. Thus, macrophages phagocytose large clumps, exceeding the bactericidal capacities of these cells. Furthermore, proinflammatory cytokines and granuloma-like structures were only produced by macrophages infected with rough morphotypes. Thus, the present study provides a foundation for further studies that consider mycobacterial clumps as virulence factors.
Matèries
Matèries (anglès)
Citació
Citació
BRAMBILLA, Cecilia, LLORENS-FONS, Marta, JULIÁN, Esther, NOGUERA ORTEGA, Estela, TOMÀS-MARTÍNEZ, Cristina, PÉREZ-TRUJILLO, Miriam, BYRD, Thomas f., ALCAIDE FERNÁNDEZ DE VEGA, Fernando, LUQUIN, Marina. Mycobacteria clumping increase their capacity to damage macrophages. _Frontiers in Microbiology_. 2016. Vol. 7, núm. 1562. [consulta: 7 de febrer de 2026]. ISSN: 1664-302X. [Disponible a: https://hdl.handle.net/2445/127523]