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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/13645
Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing
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Background: The aim was to test the hypothesis that the blood serum of rats subjected to recurrent airway obstructions mimicking obstructive sleep apnea (OSA) induces early activation of bone marrow-derived mesenchymal stem cells (MSC) and enhancement of endothelial wound healing. Methods: We studied 30 control rats and 30 rats subjected to recurrent obstructive apneas (60 per hour, lasting 15 s each, for 5 h). The migration induced in MSC by apneic serum was measured by transwell assays. MSC-endothelial adhesion induced by apneic serum was assessed by incubating fluorescent-labelled MSC on monolayers of cultured endothelial cells from rat aorta. A wound healing assay was used to investigate the effect of apneic serum on endothelial repair. Results: Apneic serum showed significant increase in chemotaxis in MSC when compared with control serum: the normalized chemotaxis indices were 2.20 +- 0.58 (m +- SE) and 1.00 +- 0.26, respectively (p < 0.05). MSC adhesion to endothelial cells was greater (1.75 +- 0.14 -fold; p < 0.01) in apneic serum than in control serum. When compared with control serum, apneic serum significantly increased endothelial wound healing (2.01 +- 0.24 -fold; p < 0.05). Conclusions: The early increases induced by recurrent obstructive apneas in MSC migration, adhesion and endothelial repair suggest that these mechanisms play a role in the physiological response to the challenges associated to OSA.
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CARRERAS, Alba, ROJAS, Mauricio, TSAPIKOUNI, Theodora, MONTSERRAT CANAL, José ma., NAVAJAS NAVARRO, Daniel, FARRÉ VENTURA, Ramon. Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing. _Respiratory Research 2010_. 11:91. Vol. 1-7. [consulta: 30 de gener de 2026]. ISSN: 1465-9921. [Disponible a: https://hdl.handle.net/2445/13645]