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2015-11-30

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BACE-1, PS-1 and sAPPβ levels are increased in plasma from sporadic inclusion body myositis patients: surrogate biomarkers among inflammatory myopathies

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https://doi.org/10.2119/molmed.2015.00168

Resum

Sporadic inclusion body myositis (sIBM) is a rare disease which is difficult to diagnose. Muscle biopsy provides three prominent pathological findings: inflammation, mitochondrial abnormalities and fibber degeneration represented by the accumulation of protein depots constituted by β-amyloid peptide, among others. We aim to perform a screening in plasma of circulating molecules related to the putative etiopathogenesis of sIBM to determine potential surrogate biomarkers for diagnosis. Plasma from 21 sIBM patients and 20 age and gender-paired healthy controls were collected and stored at -80ºC. An additional population of patients with non-sIBM inflammatory myopathies was also included (9 patients with dermatomyositis and 5 with polymyositis). Circulating levels of inflammatory cytokines (IL-6 and TNF-α), mitochondrial-related molecules (free plasmatic mtDNA, FGF-21 and CoQ) and amyloidogenic-related molecules (BACE-1, PS-1 and sAPPβ) were assessed with magnetic bead-based assays, rt-PCR, ELISA and HPLC. Despite remarkable trends towards altered plasmatic expression of inflammatory and mitochondrial molecules (increased IL-6, TNF-α, circulating mtDNA and FGF-21 levels and decreased content in CoQ), only amyloidogenic degenerative markers including BACE-1, PS-1 and sAPPβ levels were significantly increased in plasma from sIBM patients compared to controls and other patients with non-sIBM inflammatory myopathies (p<0.05). Inflammatory, mitochondrial and amyloidogenic degeneration markers are altered in plasma of sIBM patients confirming their etiopathological implication in the disease. Sensitivity and specificity analysis show that BACE-1, PS-1 and sAPPβ represent a good predictive non-invasive tool for the diagnosis of sIBM, especially in distinguishing this disease from polymyositis.

Matèries

Miositis, Biòpsia, Inflamació

Matèries (anglès)

Myositis, Biopsy, Inflammation

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Articles publicats en revistes (Medicina)

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VILLARROYA I GOMBAU, Francesc, et al. BACE-1, PS-1 and sAPPβ levels are increased in plasma from sporadic inclusion body myositis patients: surrogate biomarkers among inflammatory myopathies. Molecular Medicine. 2015. Vol. 21, num. 1, pags. 817-823. ISSN 1076-1551. [consulted: 28 of May of 2026]. Available at: https://hdl.handle.net/2445/219049

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