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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/183115
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
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The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
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RODRIGUEZ TEMPORAL, David, et al. Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification. Scientific Reports. 2022. Vol. vol 12, num. num 1. ISSN 2045-2322. [consulted: 9 of June of 2026]. Available at: https://hdl.handle.net/2445/183115