Immunostaining Protocol: P-Smad2 (Xenograft and Mice)

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dc.contributor.authorCalon, Alexandre
dc.contributor.authorEspinet, Elisa
dc.contributor.authorPalomo Ponce, Sergio
dc.contributor.authorTauriello, Daniele V. F.
dc.contributor.authorIglesias, Mar
dc.contributor.authorCéspedes, María
dc.contributor.authorSevillano, Marta
dc.contributor.authorNadal, Cristina
dc.contributor.authorJung, Peter
dc.contributor.authorZhang, Xiang
dc.contributor.authorByrom, Daniel
dc.contributor.authorRiera i Escalé, Antoni
dc.contributor.authorRossell, David
dc.contributor.authorMangues, Ramon
dc.contributor.authorMassagué i Solé, Joan, 1953-
dc.contributor.authorSancho, Elena
dc.contributor.authorBatlle, Eduard
dc.date.accessioned2022-02-24T19:00:17Z
dc.date.available2022-02-24T19:00:17Z
dc.date.issued2014-05-05
dc.date.updated2022-02-24T12:05:08Z
dc.description.abstractMetastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF- beta responses when injected into nude mice as shown by lack of p- SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β, KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells.
dc.format.extent4 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idimarina6544613
dc.identifier.issn2331-8325
dc.identifier.urihttps://hdl.handle.net/2445/183522
dc.language.isoeng
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.21769/bioprotoc.1119
dc.relation.ispartofBio-protocol, 2014, vol. 4, num. 9, p. e1119
dc.relation.urihttps://doi.org/10.21769/bioprotoc.1119
dc.rights(c) Bio-protocol LLC, 2014
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.sourceArticles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
dc.subject.classificationCàncer colorectal
dc.subject.classificationMetàstasi
dc.subject.classificationExpressió gènica
dc.subject.otherColorectal cancer
dc.subject.otherMetastasis
dc.subject.otherGene expression
dc.titleImmunostaining Protocol: P-Smad2 (Xenograft and Mice)
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion

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