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Article

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2014-05-05

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/183522

Immunostaining Protocol: P-Smad2 (Xenograft and Mice)

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https://doi.org/10.21769/bioprotoc.1119

Abstract

Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF- beta responses when injected into nude mice as shown by lack of p- SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β, KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells.

Subject

Càncer colorectal, Metàstasi, Expressió gènica

Subject (English)

Colorectal cancer, Metastasis, Gene expression

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Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Citation

CALON, Alexandre, et al. Immunostaining Protocol: P-Smad2 (Xenograft and Mice). Bio-protocol. 2014. Vol. 4, num. 9, pags. e1119. ISSN 2331-8325. [consulted: 9 of June of 2026]. Available at: https://hdl.handle.net/2445/183522

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