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2023-11-16

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cc-by (c) Marta Kovatcheva et al., 2023
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/204119

Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair

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https://doi.org/10.1038/s42255-023-00916-6

Abstract

Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration and rejuvenation, but little is known about its metabolic requirements. Here we show that OSKM reprogramming in mice causes a global depletion of vitamin B12 and molecular hallmarks of methionine starvation. Supplementation with vitamin B12 increases the efficiency of reprogramming both in mice and in cultured cells, the latter indicating a cell-intrinsic effect. We show that the epigenetic mark H3K36me3, which prevents illegitimate initiation of transcription outside promoters (cryptic transcription), is sensitive to vitamin B12 levels, providing evidence for a link between B12 levels, H3K36 methylation, transcriptional fidelity and efficient reprogramming. Vitamin B12 supplementation also accelerates tissue repair in a model of ulcerative colitis. We conclude that vitamin B12, through its key role in one-carbon metabolism and epigenetic dynamics, improves the efficiency of in vivo reprogramming and tissue repair

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Vitamines hidrosolubles, Epigenètica

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Water-soluble vitamins, Epigenetics

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Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Citation

KOVATCHEVA, Marta, et al. Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair. Nature Metabolism. 2023. Vol. 5, num. 1911-1930. ISSN 2522-5812. [consulted: 14 of August of 2026]. Available at: https://hdl.handle.net/2445/204119

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