Dentin growth after direct pulp capping with the different fractions of plasma rich in growth factors (PRGF) vs MTA: experimental study in animal model

Abstract

Background: The study aimed to evaluate the area of dentin growth in rabbit incisors after pulp capping with plasma rich in growth factors (PRGF) compared with mineral trioxide aggregate (MTA) by fluorescence. Methods: 27 upper and lower incisors of rabbits were divided into 4 groups: poor PRGF (F1) (n = 9 teeth), rich PRGF (F2) (n = 8 teeth), ProRoot MTA (positive control, n = 5 teeth) and untreated (NC) (negative control, n=5). Fluorochrome markers were injected 24 hours before surgery and the day before euthanasia, 28 days after the vital pulp therapy (VPT). Two transverse cuts were performed to every tooth: the first cut (A), 1 mm incisal to the gingival margin, and the second cut (B), 5 mm apical to the first cut. The sections were assessed with histomorphometric evaluation by fluorescence microscopy, comparing the dentin area between fluorescence marks and the total mineralized area. Results: The higher percentage of dentin growth was observed in the F2 group (B=63.25%, A=36.52%), followed by F1 (B=57.63%, A=30,12%) and MTA (B=38.64%, A=15,74%) The group with lowest percentage of dentin growth was the NC group (B=29.22%, A=7.82%). Significant difference (p <0.05) was found between F2 group and MTA, also statistically significant difference has been observed comparing dentin growth areas of NC group with F1 and F2 groups. Conclusions: The application of PRGF rich and poor fraction as a pulp capping material stimulated dentin formation significantly more intensively than MTA and NC.