Histological organisation of the pancreas in rats

dc.contributor.authorPérez Clausell, Jeús
dc.contributor.authorUniversitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia
dc.date.accessioned2025-10-15T11:46:01Z
dc.date.available2025-10-15T11:46:01Z
dc.date.issued2025
dc.description.abstract[ENG] A collection of micrographs of the pancreas in rats. General views and details of the exocrine acini, endocrine islets, secretory ducts, arteries, and veins are shown. Paraffin sections were stained with hæmatoxylin-eosin or van Gieson's trichrome, while epoxy semi-thin sections (2 µm thick) were stained with toluidine blue. This collection complements the micrographs published by Dr Elena Sagristà i Mateo → https://diposit.ub.edu/dspace/handle/2445/60217 ← See also: → https://diposit.ub.edu/dspace/browse?type=author&value=Sagrist%C3%A0+i+Mateo%2C+Elena ←ca
dc.description.abstract[CAT] Col·lecció de micrografies que mostren l'organització histològica del pàncrees de la rata i la relació amb el duodè. S’hi observa tant l'aspecte general del pàncrees com detalls dels àcins exocrins, els illots endocrins (de Langerhans), els conductes secretors, artèries i venes. S'han utilitzat talls de parafina tenyits amb hematoxilina-eosina o el tricròmic de van Gieson, així com talls semifins de 2 µm tenyits amb blau de toluïdina. Aquesta col·lecció complementa les micrografies publicades per la Dra. Elena Sagristà i Mateo → https://diposit.ub.edu/dspace/handle/2445/60217 ← See also: → https://diposit.ub.edu/dspace/browse?type=author&value=Sagrist%C3%A0+i+Mateo%2C+Elena ←ca
dc.format.extent100 imatges
dc.format.mimetypeimage/jpeg
dc.identifier.citationCite as: Pérez-Clausell J (2025) << Title>>. Universitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia. Dipòsit digital UB [consulted on <<date>>] https://hdl.handle.net/2445/??????ca
dc.identifier.urihttps://hdl.handle.net/2445/223675
dc.language.isoengca
dc.rightscc by-nc-nd (c) Pérez Clausell, Jeús, 2025
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/es/*
dc.sourceOMADO (Objectes i MAterials DOcents)
dc.subject.classificationTub digestiucat
dc.subject.classificationDuodècat
dc.subject.classificationPàncreescat
dc.subject.classificationIllots de Langerhans
dc.subject.classificationHistologia
dc.subject.classificationRates (Animals de laboratori)
dc.titleHistological organisation of the pancreas in ratsca
dc.title.alternativeOrganització histològica del pàncrees de la rataca
dc.typeinfo:eu-repo/semantics/otherca

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P_O_duodenum_HEosin_4x_47_LETTERS.jpg
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Duodenum and adjoining pancreas in a rat. The pancreatic acini are darkly stained and form discrete masses – lobules – embedded in the mesenterium (mes), which is lightly stained and joins the duodenal wall. Intralobular septa of loose connective tissue – virtually unstained – separate these acinar masses, and blood vessels – arteries (ar) and veins (ve) – lie within them. The duodenal wall exhibits the typical layers: mucosa, submucosa (sub), and muscularis externa (me). The mucosa projects into the lumen, forming villi (black arrowheads). They are lined by a single-layered epithelium and enclose a loose connective tissue: the lamina propria (lp) which is palely stained. The epithelium also invaginates into the mucosa, forming crypts. A thin muscularis mucosæ (mm) constitutes the deepest border of the mucosa. Beneath this lies the submucosa (sub), a moderately stained connective tissue layer, and then a compact muscularis externa (me) which stains heavily. Paraffin section, hæmatoxylin-eosin stain.
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P_O_duodenum_HEosin_4x_47.jpg
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Duodenum and adjoining pancreas in a rat. No labelling.
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P_O_pancreas_HEosin_4X_03_06_collage_LETTERS.jpg
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Duodenal lobe of the rat pancreas (collage of micrographs allowing moderate zoom). Pancreatic acini are darkly stained and lie in the palely stained mesenterium, which is wrapped in a thin serosa (ser). Faintly stained septa of connective tissue separate the acinar lobules. These septa accommodate large and small branches of arteries (ar) and veins (ve), which also reach the duodenal wall. In this longitudinal section of the duodenum, only its deepest layers are seen: the crypts and a thin muscularis mucosæ (mm), supported by a moderately stained submucosa (sub), followed by another smooth muscle layer – muscularis externa –. This layer can be divided into inner and outer sublayers, which are barely noticeable even when zooming in. Paraffin section, hæmatoxylin-eosin stain.
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P_O_pancreas_HEosin_4X_03_06_collage.jpg
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Duodenal lobe of the rat pancreas (collage of micrographs). No labelling.
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P_O_pancreas_HEosin_20X_07_10_collageB_LETTERS.jpg
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Duodenal wall and pancreatic lobules (collage of micrographs allowing detailed zoom). In the duodenum, villi are seen projecting from the wall and lined by a simple columnar epithelium (ep, bidirectional white arrows). Goblet cells (Go) can be seen interspersed between the absorptive cells, which are stained darker. Invaginations of the epithelium form crypts, surrounded by a pale lamina propria (lp). A thin layer of smooth muscle cells – the muscularis mucosæ (mm) – marks the inner border of the mucosa. A denser connective tissue is located underneath, forming the submucosa (sub). The muscularis externa (me) can be seen arranged into two layers: the inner or circular (ic), and the outer or longitudinal (ol). A virtually unstained mesenterium (mes) supports the pancreatic lobules and attaches them to the duodenum. Fibroblasts (fi) and delicate collagen fibres (black arrowheads) are found there. Intralobular septa (empty arrowheads) – also unstained – separate the acinar masses. In these, each acinus appears as a ring stained blue or purple with a core stained red. Acinar cell nuclei (N) are located in that peripheral ring, and are stained dark blue or purple. Arterioles (ar) and venules (v) of various sizes lie in the septa and inside the acinar masses. Paraffin section, hæmatoxylin-eosin stain.