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Accepted version

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2015-08

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/133518

Identification and characterization of the zebrafish ClC-2 chloride channel orthologs

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https://doi.org/10.1007/s00424-014-1614-z

Abstract

ClC-2 is a Cl− channel that belongs to the CLC family of chloride channel/transport proteins. ClC-2 molecular role is not clear, and Clcn2 knockout mice develop blindness, sterility, and leukodystrophy by unknown reasons. ClC-2 is associated in the brain with the adhesion molecule GlialCAM, which is defective in a type of leukodystrophy, involving ClC-2 in the homeostasis of myelin. To get more insight into the functions of ClC-2, we have identified in this work the three ClC-2 orthologs in zebrafish. clcn2a and clcn2b resulted from the teleost-specific whole genome duplication, while clcn2c arose from a gene duplication from clcn2b. The expression patterns in adult tissues and embryos of zebrafish clcn2 paralogs support their subfunctionalization after the duplications, with clcn2a being enriched in excitable tissues and clcn2c in ionocytes. All three zebrafish clc-2 proteins interact with human GLIALCAM, that is able to target them to cell junctions, as it does with mammalian ClC-2. We could detect clc-2a and clc-2b inward rectified chloride currents with different voltage-dependence and kinetics in Xenopus oocytes, while clc-2c remained inactive. Interestingly, GlialCAM proteins did not modify clc-2b inward rectification. Then, our work extends the repertoire of ClC-2 proteins and provides new tools for structure-function and physiology studies.

Subject

Canals de clorur, Seqüència de nucleòtids, Metabolisme de proteïnes, Peix zebra

Subject (English)

Chloride channels, Nucleotide sequence, Protein metabolism, Zebra danio

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Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citation

PÉREZ RIUS, Carla, et al. Identification and characterization of the zebrafish ClC-2 chloride channel orthologs. Pflugers Archiv-European Journal of Physiology. 2015. Vol. 467, num. 8, pags. 1769-1781. ISSN 0031-6768. [consulted: 12 of August of 2026]. Available at: https://hdl.handle.net/2445/133518

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