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2020-01-11

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cc-by (c) Vergara, Andrea et al., 2020
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/150965

Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia

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https://doi.org/10.3390/microorganisms8010103

Abstract

Rapid identification of the causative agent of hospital-acquired pneumonia (HAP) will allowan earlier administration of a more appropriate antibiotic and could improve the outcome of thesepatients. The aim of this study was to develop a rapid protocol to identify the main microorganismsinvolved in HAP by loop-mediated isothermal amplification (LAMP) directly from respiratory samples.First of all, a rapid procedure (<30 min) to extract the DNA from bronchoalveolar lavage (BAL),endotracheal aspirate (EA) or bronchoaspirate (BAS) was set up. A specific LAMP forStaphylococcusaureus,Escherichia coli,Klebsiella pneumoniae, Pseudomonas aeruginosa,Stenotrophomonas maltophiliaandAcinetobacter baumanniiwas performed with the extracted solution at 65◦C for 30-40 min. Overall,58 positive BAL and 83 EA/BAS samples were tested. The limits of detection varied according to themicroorganism detected. Validation of the LAMP assay with BAL samples showed that the assay was 100% specific and 86.3% sensitive (positive predictive value of 100% and a negative predictive valueof 50%) compared with culture. Meanwhile for BAS/EA samples, the assay rendered the followingstatistical parameters: 100% specificity, 94.6% sensitivity, 100% positive predictive value and 69.2%negative predictive value. The turnaround time including sample preparation and LAMP was circa1 h. LAMP method may be used to detect the most frequent bacteria causing HAP. It is a simple,cheap, sensitive, specific and rapid assay.

Subject

Diagnòstic, Medicina intensiva, Aparell respiratori, Pneumònia adquirida a la comunitat

Subject (English)

Diagnosis, Critical care medicine, Respiratory organs, Community-acquired pneumonia

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Articles publicats en revistes (Fonaments Clínics)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Medicina)

Citation

VERGARA GÓMEZ, Andrea, et al. Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia. Microorganisms. 2020. Vol. 8, num. 1, pags. 103. ISSN 2076-2607. [consulted: 13 of August of 2026]. Available at: https://hdl.handle.net/2445/150965

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