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2012-10-23

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cc by (c) Juan Mateu et al., 2012
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/126494

Prognostic value of X-chromosome inactivation in symptomatic female carriers of dystrophinopathy

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https://doi.org/10.1186/1750-1172-7-82

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Background: Between 8% and 22% of female carriers of DMD mutations exhibit clinical symptoms of variable severity. Development of symptoms in DMD mutation carriers without chromosomal rearrangements has been attributed to skewed X-chromosome inactivation (XCI) favouring predominant expression of the DMD mutant allele. However the prognostic use of XCI analysis is controversial. We aimed to evaluate the correlation between X-chromosome inactivation and development of clinical symptoms in a series of symptomatic female carriers of dystrophinopathy. Methods: We reviewed the clinical, pathological and genetic features of twenty-four symptomatic carriers covering a wide spectrum of clinical phenotypes. DMD gene analysis was performed using MLPA and whole gene sequencing in blood DNA and muscle cDNA. Blood and muscle DNA was used for X-chromosome inactivation (XCI) analysis thought the AR methylation assay in symptomatic carriers and their female relatives, asymptomatic carriers as well as non-carrier females. Results: Symptomatic carriers exhibited 49.2% more skewed XCI profiles than asymptomatic carriers. The extent of XCI skewing in blood tended to increase in line with the severity of muscle symptoms. Skewed XCI patterns were found in at least one first-degree female relative in 78.6% of symptomatic carrier families. No mutations altering XCI in the XIST gene promoter were found. Conclusions: Skewed XCI is in many cases familial inherited. The extent of XCI skewing is related to phenotype severity. However, the assessment of XCI by means of the AR methylation assay has a poor prognostic value, probably because the methylation status of the AR gene in muscle may not reflect in all cases the methylation status of the DMD gene.

Matèries

Malalties neuromusculars, Cromosomes

Matèries (anglès)

Neuromuscular diseases, Chromosomes

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Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

JUAN MATEU, Jonàs, RODRÍGUEZ, Maria josé, NASCIMENTO, Andrés, JIMÉNEZ MALLEBRERA, Cecilia, GONZÁLEZ QUEREDA, Lidia, RIVAS, Eloy, PARADAS, Carmen, MADRUGA, Marcos, SÁNCHEZ AYASO, Pedro, JOU, Cristina, GONZÁLEZ MERA, Laura, MUNELL CASADESÚS, Francina, ROIG QUILIS, Manuel, RABASA, Maria, HERNÁNDEZ LAIN, Aurelio, DÍAZ MANERA, Jordi, GALLARDO, Eduard, PASCUAL CALVET, Jordi, VERDURA, Edgard, COLOMER OFERIL, Jaume, BAIGET BASTÚS, Montserrat, OLIVÉ I PLANA, Montserrat, GALLANO, Pia. Prognostic value of X-chromosome inactivation in symptomatic female carriers of dystrophinopathy. _Orphanet Journal of Rare Diseases_. 2012. Vol. 7, núm. 82. [consulta: 23 de gener de 2026]. [Disponible a: https://hdl.handle.net/2445/126494]

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