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2022-03-07

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cc-by (c) Basto-Silva, Catarina et al., 2022
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/186297

Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality

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Recurs relacionat

https://doi.org/10.3390/fishes7020059

Resum

This study investigated, for the first time, the integrated effects of dietary protein source and protein/carbohydrate (P/CH) ratio on gilthead seabream gut histomorphology, microbiota composition, digestive enzymes activity, and immunological and oxidative stress-related gene expressions. Four isolipidic diets: two fishmeal-based (FM) and two plant feedstuff (PF)-based diets, with P/CH ratios of 50/10 or 40/20 each (FM-P50/CH10; FM-P40/CH20; PF-P50/CH10; PF-P40/CH20), were tested. PF-based diets lead to more histomorphological alterations than FM-based diets. P/CH ratio had no relevant effect on gut histomorphology. Gut mucosa of fish fed PF-based diets presented a higher number of operational taxonomic units, and richness and diversity indices, while the P/CH ratio did not affect those parameters. The α-amylase activity was lower in fish fed with PF-based diets and in fish fed the P40/CH20 diets. Regarding the immune-related genes, only cyclooxygenase-2 was affected, being higher in fish fed the P50/CH10 diets than the P40/CH20 diets. Fish fed the FM-based diets presented higher expression of glutathione reductase and glutathione peroxidase, while fish fed the P50/CH10 diet had higher expression of superoxide dismutase. In conclusion, PF-based diets can compromise gut absorptive and digestive metabolism, but decreasing the dietary P/CH ratio had little effect on the parameters measured.

Matèries

Orada, Microbiota intestinal, Expressió gènica, Estrès oxidatiu

Matèries (anglès)

Sparus aurata, Gastrointestinal microbiome, Gene expression, Oxidative stress

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Col·leccions

Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

Citació

BASTO-SILVA, Catarina, et al. Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality. Fishes. 2022. Vol. 7, num. 2, pags. 59. ISSN 2410-3888. [consulted: 19 of August of 2026]. Available at: https://hdl.handle.net/2445/186297

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