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GarciaJI_SciRep_2019.pdf (1.41 MB)

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2019-11-29

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cc by (c) García et al., 2019
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/147228

Improved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk

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http://dx.doi.org/10.1038/s41598-019-54537-9

Resum

Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with \xCE\xB1-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.

Matèries

Tuberculosi, Diagnòstic immunològic

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Tuberculosis, Immunodiagnosis

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Articles publicats en revistes (ISGlobal)

Citació

GARCÍA, Juan ignacio, KELLEY, Holden v., MELÉNDEZ, Johanna, ALVAREZ DE LEÓN, Rosa alejandra, CASTILLO, Alejandra, SIDIKI, Sabeen, YUSOOF, Kizil a., NUNES, Elizabete, LÓPEZ TÉLLEZ, Cesar, MEJÍA VILLATORO, Carlos rodolfo, IKEDA, Janet, GARCÍA-BASTEIRO, Alberto l., WANG, Shu-hua, TORRELLES, Jordi b.. Improved Alere Determine Lipoarabinomannan Antigen Detection
                Test for the Diagnosis of Human and Bovine Tuberculosis by
                Manipulating Urine and Milk. _Scientific Reports_. 2019. Vol. 9, núm. 18012. [consulta: 11 de gener de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/147228]

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