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628803.pdf (3.73 MB)

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2013-10-25

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/181261

β-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1α interaction to potentiate glutamate release at cerebrocortical nerve terminal

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https://doi.org/10.1074/jbc.M113.463877

Resum

The adenylyl cyclase activator forskolin presynaptically facilitates synaptic transmission through cAMP-dependent protein kinase, PKA. However, cAMP also increases glutamate release via PKA-independent mechanisms, although the downstream presynaptic targets remain largely unknown. Here we found that a PKA-independent release component can be isolated in cerebrocortical nerve terminals after blocking Na+ channels with tetrodotoxin. 8-pCPT-2-O-Me-cAMP, 8pCPT, a specific activator of the exchange protein directly activated by cAMP, Epac, mimicks and occluded forskolin-induced release. The Epac mediated increase in release is dependent on phospholipase C, and increased phosphatidylinositol (4,5)-bisphosphate hydrolysis. Furthermore, the potentiation of release does not depend on protein kinase C, although it is reduced by the diacylglycerol-binding site antagonist calphostin C. Epac activation translocates the active zone protein Munc13-1 from soluble to particulate fractions, increases the association between Rab3A and Rim1αand redistributes synaptic vesicles to positions closer to the presynaptic membrane. We also found that the β-adrenergic receptor agonist, isoproterenol, mimicked all these responses consistent with high-resolution immunoelectron microscopy and immunocytochemical data showing presynaptic expression of the β-ARs at a subset of glutamatergic synapses of the cerebral cortex. It is concluded that β adrenergic receptors couple to a cAMP/Epac/PLC/Munc13/Rab3/Rim dependent pathway to enhance glutamate release at cerebrocortical nerve terminals.

Matèries

Receptors adrenèrgics, Nervis, Proteïnes

Matèries (anglès)

Adrenaline receptors, Nerves, Proteins

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Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

FERRERO, Jose J., et al. β-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1α interaction to potentiate glutamate release at cerebrocortical nerve terminal. Journal of Biological Chemistry. 2013. Vol. 288, num. 43, pags. 31370-31385. ISSN 0021-9258. [consulted: 17 of August of 2026]. Available at: https://hdl.handle.net/2445/181261

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