Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/51084
Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion.
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and adult BM CD45--enriched cells. Human mesenspheres robustly differentiated into mesenchymal lineages. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded HSCs were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. These results pave the way for culture techniques that preserve the self-renewal of human BMSCs and their ability to support functional HSCs.
Matèries
Matèries (anglès)
Citació
Col·leccions
Citació
ISERN, Joan, MARTÍN-ANTONIO, Beatriz, GHAZANFARI, Roshanak, MARTÍN, Aan m., LÓPEZ, Juan a., TORO, Raquel del, SÁNCHEZ-AGUILERA, Abel, ARRANZ, Lorena, MARTÍN-PÉREZ, Daniel, SUÁREZ-LLEDÓ, María, MARÍN, Pedro, VAN PEL, Melissa, FIBBE, Willem e., VÁZQUEZ, Jesús, SCHEDING, Stefan, URBANO ISPIZUA, Álvaro, MÉNDEZ-FERRER, Simón. Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion.. _Cell Reports_. 2013. Vol. 3, núm. 5, pàgs. 1714-1724. [consulta: 20 de gener de 2026]. ISSN: 2211-1247. [Disponible a: https://hdl.handle.net/2445/51084]