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Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique
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BACKGROUND: Malaria programmes use Plasmodium falciparum
histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests
(RDTs) for malaria diagnosis. The deletion of this target
antigen could potentially lead to misdiagnosis, delayed
treatment and continuation of active transmission. METHODS:
Plasmodium falciparum isolates (n = 1162) collected in Southern
Mozambique were assessed by RDTs, microscopy and/or 18SrRNA
qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates
from individuals who were negative by RDT but positive by
microscopy and/or qPCR (n = 69) using gene-specific PCRs, with
kelch13 PCR as the parasite DNA control. RESULTS: Lack of pfhrp2
PCR amplification was observed in one of the 69 isolates
subjected to molecular analysis [1.45% (95% CI 0.3-7.8%)].
CONCLUSIONS: The low prevalence of pfhrp2 deletions suggests
that RDTs will detect the vast majority of the P. falciparum
infections. Nevertheless, active surveillance for changing
deletion frequencies is required.
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GUPTA, Himanshu, et al. Molecular surveillance of pfhrp2 and pfhrp3 deletions in
Plasmodium falciparum isolates from Mozambique. Malaria Journal. 2017. Vol. 16, num. 1, pags. 416. ISSN 1475-2875. [consulted: 4 of August of 2026]. Available at: https://hdl.handle.net/2445/117493