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Lighting up multiprotein complexes: lessons from GPCR oligomerization
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Spatiotemporal characterization of protein protein interactions (PPIs) is essential in determining the molecular mechanisms of intracellular signaling processes. In this review, we discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of G protein-coupled receptor (GPCR) oligomerization, can be used to detect specific PPIs in live cells. These technologies alone or in concert with complementary methods (SRET, BRET or BiFC, and SNAP-tag or TR-FRET) can be extremely powerful approaches for PPI visualization, even between more than two proteins. Here we provide a comprehensive update on all the biotechnological aspects, including the strengths and weaknesses, of new fluorescence- and luminescence-based methodologies, with a specific focus on their application for studying PPIs.
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CIRUELA ALFÉREZ, Francisco, VILARDAGA, Jean pierre, FERNÁNDEZ DUEÑAS, Víctor. Lighting up multiprotein complexes: lessons from GPCR oligomerization. _Trends in Biotechnology_. 2010. Vol. 28, núm. 8, pàgs. 407-415. [consulta: 22 de gener de 2026]. ISSN: 0167-7799. [Disponible a: https://hdl.handle.net/2445/126429]