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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/126429
Lighting up multiprotein complexes: lessons from GPCR oligomerization
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Spatiotemporal characterization of protein protein interactions (PPIs) is essential in determining the molecular mechanisms of intracellular signaling processes. In this review, we discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of G protein-coupled receptor (GPCR) oligomerization, can be used to detect specific PPIs in live cells. These technologies alone or in concert with complementary methods (SRET, BRET or BiFC, and SNAP-tag or TR-FRET) can be extremely powerful approaches for PPI visualization, even between more than two proteins. Here we provide a comprehensive update on all the biotechnological aspects, including the strengths and weaknesses, of new fluorescence- and luminescence-based methodologies, with a specific focus on their application for studying PPIs.
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CIRUELA ALFÉREZ, Francisco, VILARDAGA, Jean Pierre and FERNÁNDEZ DUEÑAS, Víctor. Lighting up multiprotein complexes: lessons from GPCR oligomerization. Trends in Biotechnology. 2010. Vol. 28, num. 8, pags. 407-415. ISSN 0167-7799. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/126429