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fncel-17-1057242.pdf (3.69 MB)

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Versió publicada

Data de publicació

2023-05-17

Llicència de publicació

cc by (c) Martínez San Segundo, Pablo et al, 2023
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/200835

Multivesicular release favors short term synaptic depression in hippocampal autapses

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Recurs relacionat

https://doi.org/10.3389/fncel.2023.1057242

Resum

Presynaptic terminals of the central nervous system can support univesicular and multivesicular synchronous release of neurotransmitters, however, the functional implications of the prevalence of one mechanism over the other are yet unresolved. Here, we took advantage of the expression of SF-iGluSnFR.S72A in the astrocytic feeder layer of autaptic hippocampal neuronal cultures to associate the liberation of glutamate to excitatory postsynaptic currents. The presence of the glutamate sensor in glial cells avoided any interference with the function of endogenous postsynaptic receptors. It was possible to optically detect changes in neurotransmitter release probability, which was heterogeneous among synaptic boutons studied. For each neuron investigated, the liberation of neurotransmitters occurred through a predominant mechanism. The prevalence of multivesicular over univesicular release increased synaptic strength and enhanced short-term synaptic depression. These results show that the preference of hippocampal boutons to synchronously release one or more vesicles determines the strength and low pass filtering properties of the synapses established.

Matèries

Sinapsi, Receptors de neurotransmissors

Matèries (anglès)

Synapses, Neurotransmitter receptors

Citació

Col·leccions

Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

MARTÍNEZ SAN SEGUNDO, Pablo, TERNI, Beatrice and LLOBET BERENGUER, Artur. Multivesicular release favors short term synaptic depression in hippocampal autapses. Frontiers in Cellular Neuroscience. 2023. Vol. 17. ISSN 1662-5102.. [consulted: 4 of August of 2026]. Available at: https://hdl.handle.net/2445/200835

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