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jcm-11-05077-v3.pdf (720.48 KB)

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2022-08-29

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cc by (c) Resina, Elena et al., 2022
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/189565

Evaluation of a New Monoclonal Chemiluminescent Immunoassay Stool Antigen Test for the Diagnosis of Helicobacter pylori Infection: A Spanish Multicentre Study

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https://doi.org/10.3390/jcm11175077

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The stool antigen test (SAT) represents an attractive alternative for detection of Helicobacter pylori. The aim of this study was to assess the accuracy of a new SAT, the automated LIAISON(R) Meridian H. pylori SA based on monoclonal antibodies, compared to the defined gold standard C-13-urea breath test (UBT). This prospective multicentre study (nine Spanish centres) enrolled patients >= 18 years of age with clinical indication to perform UBT for the initial diagnosis and for confirmation of bacterial eradication. Two UBT methods were used: mass spectrometry (MS) including citric acid (CA) or infrared spectrophotometry (IRS) without CA. Overall, 307 patients (145 naive, 162 with confirmation of eradication) were analysed. Using recommended cut-off values (negative SAT < 0.90, positive >= 1.10) the sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 67%, 97%, 86%, 92% and 91%, respectively, obtaining an area under the receiver operating characteristic (ROC) curve (AUC) of 0.85. Twenty-eight patients, including seven false positives and 21 false negatives, presented a discordant result between SAT and UBT. Among the 21 false negatives, four of six tested with MS and 11 of 15 tested with IRS presented a borderline UBT delta value. In 25 discordant samples, PCR targeting H. pylori DNA was performed to re-assess positivity and SAT accuracy was re-analysed: sensitivity, specificity, positive predictive value, negative predictive value, accuracy and AUC were 94%, 97%, 86%, 99%, 97% and 0.96, respectively. The new LIAISON(R) Meridian H. pylori SA SAT showed a good accuracy for diagnosis of H. pylori infection.

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Helicobacter pylori, Diagnòstic

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Helicobacter pylori, Diagnosis

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Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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RESINA, Elena, DONDAY, María g., MARTÍNEZ DOMÍNGUEZ, Samuel j., LASERNA MENDIETA, Emilio josé, LANAS, Ángel, LUCENDO, Alfredo j., SÁNCHEZ LUENGO, Marta, ALCAIDE, Noelia, FERNÁNDEZ SALAZAR, Luis, DE LA PEÑA NEGRO, Luisa, BUJANDA, Luis, ARBULO, Marta gómez ruiz de, ALCEDO, Javier, PÉREZ AISA, Ángeles, RODRÍGUEZ, Raúl, HERMIDA, Sandra, BRENES, Yanire, NYSSEN, Olga p., GISBERT, Javier p.. Evaluation of a New Monoclonal Chemiluminescent Immunoassay Stool Antigen Test for the Diagnosis of Helicobacter pylori Infection: A Spanish Multicentre Study. _Journal of Clinical Medicine_. 2022. Vol. 11, núm. 17, pàgs. 5077. [consulta: 2 de febrer de 2026]. ISSN: 2077-0383. [Disponible a: https://hdl.handle.net/2445/189565]

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