Miniatura

Fitxers

messina2018_2967.pdf (2.08 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2018-03-01

Llicència de publicació

cc by (c) Messina et al., 2018
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/121300

Gametocytes of the Malaria Parasite Plasmodium falciparum Interact With and Stimulate Bone Marrow Mesenchymal Cells to Secrete Angiogenetic Factors

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

http://dx.doi.org/10.3389/fcimb.2018.00050

Resum

The gametocytes of Plasmodium falciparum, responsible for the transmission of this malaria parasite from humans to mosquitoes, accumulate and mature preferentially in the human bone marrow. In the 10 day long sexual development of P. falciparum, the immature gametocytes reach and localize in the extravascular compartment of this organ, in contact with several bone marrow stroma cell types, prior to traversing the endothelial lining and re-entering in circulation at maturity. To investigate the host parasite interplay underlying this still obscure process, we developed an in vitro tridimensional co-culture system in a Matrigel scaffold with P. falciparum gametocytes and self-assembling spheroids of human bone marrow mesenchymal cells (hBM-MSCs). Here we show that this co-culture system sustains the full maturation of the gametocytes and that the immature, but not the mature, gametocytes adhere to hBM-MSCs via trypsin-sensitive parasite ligands exposed on the erythrocyte surface. Analysis of a time course of gametocytogenesis in the co-culture system revealed that gametocyte maturation is accompanied by the parasite induced stimulation of hBM-MSCs to secrete a panel of 14 cytokines and growth factors, 13 of which have been described to play a role in angiogenesis. Functional in vitro assays on human bone marrow endothelial cells showed that supernatants from the gametocyte mesenchymal cell co-culture system enhance ability of endothelial cells to form vascular tubes. These results altogether suggest that the interplay between immature gametocytes and hBM-MSCs may induce functional and structural alterations in the endothelial lining of the human bone marrow hosting the P. falciparum transmission stages.

Matèries

Malària, Malalties parasitàries

Matèries (anglès)

Malaria, Parasitic diseases

Citació

Col·leccions

Articles publicats en revistes (ISGlobal)

Citació

MESSINA, Valeria, VALTIERI, Mauro, RUBIO, Mercedes, FALCHI, Mario, MANCINI, Francesca romana, MAYOR APARICIO, Alfredo gabriel, ALANO, Pietro, SILVESTRINI, Francesco. Gametocytes of the Malaria Parasite Plasmodium falciparum
                Interact With and Stimulate Bone Marrow Mesenchymal Cells to
                Secrete Angiogenetic Factors. _Frontiers in Cellular and Infection Microbiology_. 2018. Vol. 8, núm. 50. [consulta: 1 de febrer de 2026]. ISSN: 2235-2988. [Disponible a: https://hdl.handle.net/2445/121300]

Exportar metadades

JSON - METS

Compartir registre