Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/101808
PTEN mediates Notch-dependent stalk cell arrest in angiogenesis.
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Coordinated activity of VEGF and Notch signals guides the endothelial cell (EC) specification into tip and stalk cells during angiogenesis. Notch activation in stalk cells leads to proliferation arrest via an unknown mechanism. By using gain- and loss-of-function gene-targeting approaches, here we show that PTEN is crucial for blocking stalk cell proliferation downstream of Notch, and this is critical for mouse vessel development. Endothelial deletion of PTEN results in vascular hyperplasia due to a failure to mediate Notch-induced proliferation arrest. Conversely, overexpression of PTEN reduces vascular density and abrogates the increase in EC proliferation induced by Notch blockade. PTEN is a lipid/protein phosphatase that also has nuclear phosphatase-independent functions. We show that both the catalytic and non-catalytic APC/C-Fzr1/Cdh1-mediated activities of PTEN are required for stalk cells' proliferative arrest. These findings define a Notch-PTEN signalling axis as an orchestrator of vessel density and implicate the PTEN-APC/C-Fzr1/Cdh1 hub in angiogenesis.
Matèries
Matèries (anglès)
Citació
Citació
SERRA, Helena, CHIVITE, Íñigo, ANGULO AGUADO, Ana, SOLER, Adriana, SUTHERLAND, James david, ARRUABARRENA-ARISTORENA, Amaia, RAGAB, Anan, LIM, Radiance, MALUMBRES, Marcos, FRUTTIGER, Marcus, POTENTE, Michael, SERRANO MARUGÁN, Manuel, FABRA FRES, Àngels, VIÑALS CANALS, Francesc, CASANOVAS I CASANOVAS, Oriol, PANDOLFI, Pier paolo, BIGAS SALVANS, Anna, CARRACEDO, Arkaitz, GERHARDT, Holger, GRAUPERA I GARCIA-MILÀ, Mariona. PTEN mediates Notch-dependent stalk cell arrest in angiogenesis.. _Nature Communications_. 2015. Vol. 6, núm. 7935. [consulta: 14 de gener de 2026]. ISSN: 2041-1723. [Disponible a: https://hdl.handle.net/2445/101808]