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2023-06-02

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cc-by-nc-nd (c) Balsells Vives, Sara et al., 2023
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/217251

Improving In Vitro Detection of Sensitization to Lipid Transfer Proteins: A New Molecular Multiplex IgE Assay

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Recurs relacionat

https://doi.org/10.1002/mnfr.202200906

Resum

Scope: LTP-syndrome is characterized by sensitization (IgE) to multiple non-specific lipid transfer proteins (nsLTPs) with a variable clinical outcome. The treatment is primarily based on offending food avoidance. However, the determination of Pru p 3-specific IgE is currently the main diagnostic tool to assess sensitization to nsLTPs. Herein, the study evaluates improvement of LTP-syndrome diagnosis and clinical management using a new IgE multiplex-immunoblot assay with a high diversity of food nsLTPs. Methods and results: An EUROLINE-LTP strip with 28 recombinant nsLTPs from 18 allergenic sources is designed. In total the study investigates 38 patients with LTP-syndrome and compares results from the nsLTPs (LTP-strip) with the respective food extracts of Prick-by-prick (PbP) testing. The agreement exceeds 70% for most nsLTPs, e.g., Pru p 3 (100%), Mal d 3 (97%), Pru av 3 (89%), Pha v 3 isoforms (87%/84%), Ara h 9 (82%), Cor a 8 (82%), and Jug r 3 (82%). The functionality and allergenic relevance of nine recombinant nsLTPs are proven by Basophil activation testing (BAT). Conclusions: The new IgE multiplex-immunoblot nsLTP assay shows a good diagnostic performance allowing culprit food assessment. Negative results from LTP-strip may indicate potentially tolerable foods, improving diet intervention and patients' quality of life.

Matèries

Proteïnes vegetals, Immunoglobulina E, Al·lèrgia alimentària, Lípids, Antígens

Matèries (anglès)

Plant proteins, Immunoglobulin E, Food allergy, Lipids, Antigens

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Articles publicats en revistes (Medicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

Citació

BALSELLS VIVES, Sara, FLUEGGE, Ulrike, BRIX, Bettina, WEIMANN, Yvonne, PERALTA, Teresa, SAN BARTOLOMÉ, Clara, ARAUJO SÁNCHEZ, Giovanna, CASAS SAUCEDO, Rocio, TORRADEFLOT, María, LARA, Rocío, MUNOZ CANO, Rosa, BARTRA TOMÀS, Joan, SUER, Waltraud, PASCAL, Mariona. Improving In Vitro Detection of Sensitization to Lipid Transfer Proteins: A New Molecular Multiplex IgE Assay. _Molecular Nutrition & Food Research_. 2023. Vol. 67, núm. 14. [consulta: 2 de febrer de 2026]. ISSN: 1613-4125. [Disponible a: https://hdl.handle.net/2445/217251]

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